Anti-PIWIL1 antibody
| Name | Anti-PIWIL1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab85125 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human, Mouse |
| Antigen | 16 amino acid peptide from the internal residues of Human PIWIL1 (NM_004764) |
| Description | Rabbit Polyclonal |
| Gene | PIWIL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PIWIL1 antibody (ab85125) at 1/1200 dilution + Human testis lysate
Cytoplasmic staining of formalin-fixed paraffin-embedded testis tissue using 1/200 ab85125.
ICC/IF image of ab85125 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.