Anti-PKC beta 1 (phospho T642) antibody
| Name | Anti-PKC beta 1 (phospho T642) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5782 |
| Prices | $370.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic phosphopeptide derived from a region of human PKC beta 1 that contains threonine 642 |
| Description | Rabbit Polyclonal |
| Gene | PRKCB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (ë) phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5782 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to PKC beta I [pT642] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12516_ab5782_1.jpg)
Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (ë) phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5782 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to PKC beta I [pT642] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
Product References
Phospholipase C and protein kinase C-beta 2 mediate insulin-like growth factor - Phospholipase C and protein kinase C-beta 2 mediate insulin-like growth factor
El-Shewy HM, Abdel-Samie SA, Al Qalam AM, Lee MH, Kitatani K, Anelli V, Jaffa AA, Obeid LM, Luttrell LM. Mol Endocrinol. 2011 Dec;25(12):2144-56.
Upregulation of osteopontin gene expression in diabetic rat proximal tubular - Upregulation of osteopontin gene expression in diabetic rat proximal tubular
Hsieh TJ, Chen R, Zhang SL, Liu F, Brezniceanu ML, Whiteside CI, Fantus IG, Ingelfinger JR, Hamet P, Chan JS. Kidney Int. 2006 Mar;69(6):1005-15.