Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]

• Supplier: Abcam
• Catalog: ab133602
• Prices: $99.00, $387.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6141
• Applications: WB IHC-P ICC/IF ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to a region within the extracellular domain of Human Placental alkaline phosphatase (PLAP)
• Description: Rabbit Monoclonal
• Gene: ALPP
• Conjugate: Unconjugated

Product images

All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/10000 dilutionLane 1 : Human placenta tissue lysateLane 2 : Human seminoma tissue lysateLane 3 : HeLa cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human dysgerminoma tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

ab133602 showing negative staining in Normal brain tissue.

ab133602 showing negative staining in Normal liver tissue.

ab133602 showing positive staining in Seminoma tissue.

ab133602 showing positive staining in Normal placenta tissue.

ab133602 showing negative staining in Normal colon tissue.

ab133602 showing negative staining in Normal tonsil tissue.

ab133602 staining Placental alkaline phosphatase (PLAP) in transgenic Mouse RIPCrez/HPAP+/+ pancreas cells (adding human placental alkaline phosphatase reporter protein to ~85% of insulin expressing beta cells) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 100% Sniper for 8 minutes at 21°C; antigen retrieval in 10mM citrate buffer for 20 minutes at 95°C. Samples were incubated with primary antibody (1/100) for 8 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD