All lanes : Anti-Plakophilin 1 antibody [EPR14890] (ab183512) at 1/20000 dilutionLane 1 : Mouse skin cell lysateLane 2 : A431 cell lysateLane 3 : HACAT cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Plakophilin 1 antibody [EPR14890] (ab183512) at 1/5000 dilutionLane 1 : Human skin tissue lysateLane 2 : Human tonsil tissue lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunofluorescent staining of 4% paraformaldehyde fixed HACAT cells labeling Plakophilin 1 using ab183512 at a 1/100 dilution followed by Goat anti Rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution. Counterstained with Dapi (bue).
Flow cytometric analysis of HACAT cells (2% paraformaldehyde fixed) labeling Plakophilin 1 with ab183512 at 1/90 dilution (red) compared to a Rabbit monoclonal isotype control (green).