Anti-Plasminogen antibody
| Name | Anti-Plasminogen antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab6189 |
| Prices | $0.00 |
| Sizes | 100 µl |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP ELISA Conjugation |
| Species Reactivities | Rat, Human |
| Antigen | Plasminogen from human plasma |
| Description | Goat Polyclonal |
| Gene | PLG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Plasminogen antibody (ab6189) at 1/5000 dilutionLane 1 : 30ug Rat plasma cell lysateLane 2 : 30ug Rat plasma cell lysateSecondaryHRP conjugated rabbit anti-goat antibodydeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 95 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteSee Abreview
Anti-Plasminogen antibody (ab6189) at 1/5000 dilution + Active human Plasminogen full length protein (ab77879) at 0.1 µgSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
ab6189 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6189 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat - Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat
Zhao M, Li M, Li X, Shao C, Yin J, Gao Y. Proteome Sci. 2014 Jul 21;12:42.
Bacillus anthracis interacts with plasmin(ogen) to evade C3b-dependent innate - Bacillus anthracis interacts with plasmin(ogen) to evade C3b-dependent innate
Chung MC, Tonry JH, Narayanan A, Manes NP, Mackie RS, Gutting B, Mukherjee DV, Popova TG, Kashanchi F, Bailey CL, Popov SG. PLoS One. 2011 Mar 25;6(3):e18119.
Plasminogen and plasmin in Alzheimer's disease. - Plasminogen and plasmin in Alzheimer's disease.
Barker R, Love S, Kehoe PG. Brain Res. 2010 Oct 8;1355:7-15.
Plasmin in nephrotic urine activates the epithelial sodium channel. - Plasmin in nephrotic urine activates the epithelial sodium channel.
Svenningsen P, Bistrup C, Friis UG, Bertog M, Haerteis S, Krueger B, Stubbe J, Jensen ON, Thiesson HC, Uhrenholt TR, Jespersen B, Jensen BL, Korbmacher C, Skott O. J Am Soc Nephrol. 2009 Feb;20(2):299-310.