All lanes : Anti-PLEKHA3 antibody [2D1] (ab117849) at 1/2000 dilutionLane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY PLEKHA3 cDNA for 48 hrs Lysates/proteins at 35 µg per lane.
Immunofluorescent staining of HeLa cells using ab117849 at a 1/100 dilution.
Flow cytometry image showing ab117849 at a 1/100 dilution, staining HEK293T cells transfected with PLEKHA3 (Red), and an empty vector control plasmid (Blue).
Flow cytometry image staining HeLa cells, using ab117849 at a 1/100 dilution (Red), and a nonspecific negative control antibody (Blue).
Flow cytometry image staining Jurkat cells, using ab117849 at a 1/100 dilution (Red), and a nonspecific negative control antibody (Blue).