Anti-Plexin A4 antibody
| Name | Anti-Plexin A4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39350 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse Plexin A4 |
| Blocking Peptide | Mouse Plexin A4 peptide |
| Description | Rabbit Polyclonal |
| Gene | PLXNA4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Plexin A4 antibody (ab39350) at 1 µg/mlLane 1 : Brain (Mouse) Tissue Lysate, blocked with 3% milkLane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)Lane 3 : Brain (Rat) Tissue Lysate, blocked with 3% milkLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/35000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Performed under reducing conditions.
ICC/IF image of ab39350 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39350, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab39350 staining Plexin A4 in mouse brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. See Abreview
Product References
Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic - Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic
Yamashita N, Usui H, Nakamura F, Chen S, Sasaki Y, Hida T, Suto F, Taniguchi M, Takei K, Goshima Y. Nat Commun. 2014 Mar 6;5:3424.
Distinct cytoplasmic domains in Plexin-A4 mediate diverse responses to semaphorin - Distinct cytoplasmic domains in Plexin-A4 mediate diverse responses to semaphorin
Mlechkovich G, Peng SS, Shacham V, Martinez E, Gokhman I, Minis A, Tran TS, Yaron A. Sci Signal. 2014 Mar 11;7(316):ra24.
Gene expression analysis of the embryonic subplate. - Gene expression analysis of the embryonic subplate.
Oeschger FM, Wang WZ, Lee S, Garcia-Moreno F, Goffinet AM, Arbones ML, Rakic S, Molnar Z. Cereb Cortex. 2012 Jun;22(6):1343-59.
PlexinA4 distribution in the adult rat spinal cord and dorsal root ganglia. - PlexinA4 distribution in the adult rat spinal cord and dorsal root ganglia.
Gutekunst CA, Stewart EN, Franz CK, English AW, Gross RE. J Chem Neuroanat. 2012 May;44(1):1-13.
Immunohistochemical Distribution of PlexinA4 in the Adult Rat Central Nervous - Immunohistochemical Distribution of PlexinA4 in the Adult Rat Central Nervous
Gutekunst CA, Stewart EN, Gross RE. Front Neuroanat. 2010 Jul 13;4. pii: 25.
Flotillin-mediated endocytic events dictate cell type-specific responses to - Flotillin-mediated endocytic events dictate cell type-specific responses to
Carcea I, Ma'ayan A, Mesias R, Sepulveda B, Salton SR, Benson DL. J Neurosci. 2010 Nov 10;30(45):15317-29.