Anti-PML Protein antibody
| Name | Anti-PML Protein antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31101 |
| Prices | $379.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: PSPSPTERAPASEEE , corresponding to amino acids 37-51 of Human PML Protein Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | PML |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab31101 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31101 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab31101 at a 1/100 dilution staining human PML in human lmyph node tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.