All lanes : Anti-PMM1 antibody [EPR17844-44] (ab202059) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-PMM1 antibody [EPR17844-44] (ab202059) at 1/2000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal heart lysateLane 3 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Anti-PMM1 antibody [EPR17844-44] (ab202059) at 1/1000 dilution + Mouse brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-PMM1 antibody [EPR17844-44] (ab202059) at 1/2000 dilutionLane 1 : Mouse spleen lysateLane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 3 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution