Anti-PMP22 antibody
| Name | Anti-PMP22 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15506 |
| Prices | $382.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)(Human) |
| Description | Rabbit Polyclonal |
| Gene | PMP22 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab155006 stained SKNSH cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15506 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Immunostaining of PMP22 in human brain (multiple sclerosis sample) using ab15506 (1:200). Secondary antibody: Goat anti rabbit Alexa488.
Immunostaining of PMP22 in human spinal cord (multiple sclerosis sample) using ab15506 (1:2000) and chromogenic detection with ABC amplification.
ab15506 staining PMP22 in human pancreas by Immunohistochemistry (FFPE-sections).
Product References
Quantitative and integrative proteome analysis of peripheral nerve myelin - Quantitative and integrative proteome analysis of peripheral nerve myelin
Patzig J, Jahn O, Tenzer S, Wichert SP, de Monasterio-Schrader P, Rosfa S, Kuharev J, Yan K, Bormuth I, Bremer J, Aguzzi A, Orfaniotou F, Hesse D, Schwab MH, Mobius W, Nave KA, Werner HB. J Neurosci. 2011 Nov 9;31(45):16369-86.
The dorsal root ganglion in Friedreich's ataxia. - The dorsal root ganglion in Friedreich's ataxia.
Koeppen AH, Morral JA, Davis AN, Qian J, Petrocine SV, Knutson MD, Gibson WM, Cusack MJ, Li D. Acta Neuropathol. 2009 Dec;118(6):763-76.
Whole-genome expression profiling of the melanoma progression pathway reveals - Whole-genome expression profiling of the melanoma progression pathway reveals
Smith AP, Hoek K, Becker D. Cancer Biol Ther. 2005 Sep;4(9):1018-29. Epub 2005 Sep 9.