Anti-PMS2 antibody [EPR3947]
| Name | Anti-PMS2 antibody [EPR3947] |
|---|---|
| Supplier | Abcam |
| Catalog | ab110638 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3947 |
| Applications | WB IP IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PMS2 aa 1-100 |
| Description | Rabbit Monoclonal |
| Gene | PMS2CL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab110638 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110638, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-PMS2 antibody [EPR3947] (ab110638) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : SH SY5Y cell lysateLane 4 : SKBR3 cell lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/13847_PMS2-Primary-antibodies-ab110638-1.gif)
All lanes : Anti-PMS2 antibody [EPR3947] (ab110638) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : SH SY5Y cell lysateLane 4 : SKBR3 cell lysateLysates/proteins at 10 µg per lane.
ab110638 at 1/100 dilution staining PMS2 in Human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
ab110638 at 1/100 dilution staining PMS2 in HeLa cells by Immunofluorescence.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD
Product References
Functional analysis of MSH2 unclassified variants found in suspected Lynch - Functional analysis of MSH2 unclassified variants found in suspected Lynch
Wielders EA, Hettinger J, Dekker R, Kets CM, Ligtenberg MJ, Mensenkamp AR, van den Ouweland AM, Prins J, Wagner A, Dinjens WN, Dubbink HJ, van Hest LP, Menko F, Hogervorst F, Verhoef S, te Riele H. J Med Genet. 2014 Apr;51(4):245-53.
Heterogenous mismatch-repair status in colorectal cancer. - Heterogenous mismatch-repair status in colorectal cancer.
Joost P, Veurink N, Holck S, Klarskov L, Bojesen A, Harbo M, Baldetorp B, Rambech E, Nilbert M. Diagn Pathol. 2014 Jun 26;9:126.
MMR deficiency is common in high-grade endometrioid carcinomas and is associated - MMR deficiency is common in high-grade endometrioid carcinomas and is associated
Nelson GS, Pink A, Lee S, Han G, Morris D, Ogilvie T, Duggan MA, Kobel M. Gynecol Oncol. 2013 Nov;131(2):309-14.
GLI1 interferes with the DNA mismatch repair system in pancreatic cancer through - GLI1 interferes with the DNA mismatch repair system in pancreatic cancer through
Inaguma S, Riku M, Hashimoto M, Murakami H, Saga S, Ikeda H, Kasai K. Cancer Res. 2013 Dec 15;73(24):7313-23.