To detect Rat EGF by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat EGF (38-211) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected rat brain (mammillary nuclei) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.