All lanes : Anti-PNMT antibody [1F3] (ab119024) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PNMT cDNALysates/proteins at 5 µg per lane.
ab119024, at 1/100 dilution, staining PNMT in COS7 cells transiently transfected with pCMV6-ENTRY PNMT by Immunofluorescence.
HEK293T cells transfected with either a PNMT overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119024 at 1/100 dilution and then analyzed by flow cytometry.
Flow cytometric analysis of PNMT in Jurkat cells using ab119024 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).