Anti-Podoplanin / gp36 antibody [18H5]
| Name | Anti-Podoplanin / gp36 antibody [18H5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab10288 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 18H5 |
| Applications | IHC-P ICC/IF ICC/IF WB FC IHC-F |
| Species Reactivities | Human |
| Antigen | Full length protein corresponding to Podoplanin/ gp36 |
| Description | Mouse Monoclonal |
| Gene | PDPN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab10288 staining Podoplanin / gp36 in human differentiated cardiac ventricular cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/300) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated goat monoclonal anti-mouse IgG (1/1500) was used as the secondary antibody.Gating Strategy: Isotype negative control (white).See Abreview
ab10288 at a 1/500 dilution staining human dermal lymphatic endothelial by Immunocytochemistry. The cells were grown in coverslips and fixed in formalin prior to permeabilization with tritonX-100. The cells were incubated with the primary antibody at 1/500 for 1 hour and then bound antibody was detected using a biotinylated goat anti-mouse antibody. Texas red-streptavidin was used for development.This image is courtesy of an Abreview submitted by Maria Navarro.See Abreview
ab10288 at 1/500 dilution staining human thymus specimen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat induced epitope retrieval step was carried out. The tisse was then blocked and incubated with the antibody. An HRP conjugated rabbit antibody was used as the secondary.See Abreview
![Overlay histogram showing HCT116 cells stained with ab10288 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10288, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14031_Podoplanin-gp36-Primary-antibodies-ab10288-2.jpg)
Overlay histogram showing HCT116 cells stained with ab10288 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10288, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
![All lanes : Anti-Podoplanin / gp36 antibody [18H5] (ab10288) at 5 µg/mlLane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 2 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14032_Podoplanin-gp36-Primary-antibodies-ab10288-4.jpg)
All lanes : Anti-Podoplanin / gp36 antibody [18H5] (ab10288) at 5 µg/mlLane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 2 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunocytochemistry/Immunofluorescence analysis of human dermal lymphatic endothelial cells (HDLEC) labelling Podoplanin / gp36 with ab10288. An Alexa Fluor® 488-conjugated goat anti-mouse was used as the secondary antibody. Nuclei counterstained with DAPI.
Product References
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Podoplanin-positive fibroblasts enhance lung adenocarcinoma tumor formation: - Podoplanin-positive fibroblasts enhance lung adenocarcinoma tumor formation:
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CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ - CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+
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Lymphatic vessels identified with podoplanin. Comparison of immunostaining with - Lymphatic vessels identified with podoplanin. Comparison of immunostaining with
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Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to - Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to
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