Anti-POLD3 antibody [2A1C11] - Azide free

Name	Anti-POLD3 antibody [2A1C11] - Azide free
Supplier	Abcam
Catalog	ab179906
Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone	2A1C11
Applications	WB IP
Species Reactivities	Human
Antigen	Recombinant full length protein corresponding to Human POLD3
Description	Mouse Monoclonal
Gene	POLD3
Conjugate	Unconjugated
Supplier Page	Shop

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Anti-POLD3 antibody [2A1C11] - Azide free (ab179906) at 1/2000 dilution + MCF7 cell extract

$Immunoprecipitation of POLD3 using ab179906. Lane 1: S100 extract of Human 293 cells (37.5 μg) was analyzed by western blotting with anti-p125, ab179906 and anti-p50 antibodies. Lanes 2-3: Coimmunoprecipitation of DNA polymerase delta subunits from the S100 extract using ab179906-bound protein A Sepharose beads. The antibody-bound proteins were eluted and detected by western blotting with respective antibodies. Eluted fractions were 2.5 μl and 5 μl out of 10 μl eluted fraction. Lane 4: eluted fraction (10 μl) from antibody-free protein A beads as a negative control.$ Immunoprecipitation of POLD3 using ab179906. Lane 1: S100 extract of Human 293 cells (37.5 μg) was analyzed by western blotting with anti-p125, ab179906 and anti-p50 antibodies. Lanes 2-3: Coimmunoprecipitation of DNA polymerase delta subunits from the S100 extract using ab179906-bound protein A Sepharose beads. The antibody-bound proteins were eluted and detected by western blotting with respective antibodies. Eluted fractions were 2.5 μl and 5 μl out of 10 μl eluted fraction. Lane 4: eluted fraction (10 μl) from antibody-free protein A beads as a negative control.