Anti-POMC antibody
| Name | Anti-POMC antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab140914 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F |
| Species Reactivities | Rat |
| Antigen | Synthetic peptide corresponding to Rat POMC aa 150 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | Pomc |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of POMC staining in Rat Brain (Arcuate Nucleus of the Hypothalamus) formalin fixed frozen tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140914, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-POMC antibody (ab140914) at 1 µg/mlLane 1 : Brain (Rat) Tissue LysateLane 2 : Cerebellum Rat Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.