Anti-PON1 antibody [17A12]
| Name | Anti-PON1 antibody [17A12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24261 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 17A12 |
| Applications | ICC/IF ICC/IF IHC-F ELISA WB FC |
| Species Reactivities | Mouse, Rat, Human, Monkey, Rabbit |
| Antigen | His tagged recombinant Human PON1 protein purified E |
| Description | Mouse Monoclonal |
| Gene | PON1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-PON1 antibody [17A12] (ab24261) at 1/500 dilutionLane 1 : HeLa cell lysates.Lane 2 : HepG2 cell lysates.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14452_Ab24261_1.JPG)
All lanes : Anti-PON1 antibody [17A12] (ab24261) at 1/500 dilutionLane 1 : HeLa cell lysates.Lane 2 : HepG2 cell lysates.
ab24261 (1µg/ml) staining PON1 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of hepatocytes.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
![Overlay histogram showing HepG2 cells stained with ab24261 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24261, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14454_PON1-Primary-antibodies-ab24261-3.jpg)
Overlay histogram showing HepG2 cells stained with ab24261 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24261, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ICC/IF image of ab24261 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24261, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-PON1 antibody [17A12] (ab24261) at 1/500 dilution + Human PON1 full length protein (ab53376) at 0.01 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/500 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14456_PON1-Primary-antibodies-ab24261-5.jpg)
Anti-PON1 antibody [17A12] (ab24261) at 1/500 dilution + Human PON1 full length protein (ab53376) at 0.01 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/500 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
Product References
Relation of atrial fibrillation (AF) and change of lipoproteins: male patients - Relation of atrial fibrillation (AF) and change of lipoproteins: male patients
Kim SM, Kim JM, Shin DG, Kim JR, Cho KH. Clin Biochem. 2014 Jul;47(10-11):869-75.
Investigation of ovarian cancer associated sialylation changes in N-linked - Investigation of ovarian cancer associated sialylation changes in N-linked
Shetty V, Hafner J, Shah P, Nickens Z, Philip R. Clin Proteomics. 2012 Aug 2;9(1):10.
CHO cell expression, long-term stability, and primate pharmacokinetics and brain - CHO cell expression, long-term stability, and primate pharmacokinetics and brain
Boado RJ, Hui EK, Lu JZ, Pardridge WM. Biotechnol Bioeng. 2011 Jan;108(1):186-96.
A homogeneous cell-based assay for measurement of endogenous paraoxonase 1 - A homogeneous cell-based assay for measurement of endogenous paraoxonase 1
Ahmad S, Carter JJ, Scott JE. Anal Biochem. 2010 May 1;400(1):1-9.
Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits - Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits
Fu AL, Wu SP. Biochem Biophys Res Commun. 2010 Jun 25;397(2):257-62. doi:
Expression and activity of paraoxonase 1 in human cataractous lens tissue. - Expression and activity of paraoxonase 1 in human cataractous lens tissue.
Hashim Z, Ilyas A, Saleem A, Salim A, Zarina S. Free Radic Biol Med. 2009 Apr 15;46(8):1089-95. doi:
Drosophila are protected from Pseudomonas aeruginosa lethality by transgenic - Drosophila are protected from Pseudomonas aeruginosa lethality by transgenic
Stoltz DA, Ozer EA, Taft PJ, Barry M, Liu L, Kiss PJ, Moninger TO, Parsek MR, Zabner J. J Clin Invest. 2008 Sep;118(9):3123-31.
Paraoxonase-2 deficiency enhances Pseudomonas aeruginosa quorum sensing in murine - Paraoxonase-2 deficiency enhances Pseudomonas aeruginosa quorum sensing in murine
Stoltz DA, Ozer EA, Ng CJ, Yu JM, Reddy ST, Lusis AJ, Bourquard N, Parsek MR, Zabner J, Shih DM. Am J Physiol Lung Cell Mol Physiol. 2007 Apr;292(4):L852-60. Epub 2006 Nov 22.