Anti-PP2A-alpha (methyl L309) antibody [2A10]
| Name | Anti-PP2A-alpha (methyl L309) antibody [2A10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab66597 |
| Prices | $400.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2A10 |
| Applications | WB ELISA IP |
| Species Reactivities | Mouse, Rat, Human, Yeast |
| Antigen | Synthetic peptide corresponding to Human PP2A-alpha conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Mouse Monoclonal |
| Gene | PPP2CA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-PP2A-alpha (methyl L309) antibody [2A10] (ab66597) at 10 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14637_PP2A-alpha-Primary-antibodies-ab66597-10.jpg)
All lanes : Anti-PP2A-alpha (methyl L309) antibody [2A10] (ab66597) at 10 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Anti-PP2A-alpha (methyl L309) antibody [2A10] (ab66597) at 1/100 dilution + Lystae prepared from mouse embryonic fibroblast cells at 25 µgSecondaryHRP-conjugatedsheep polyclonal to mouse IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14638_PP2A-alpha-Primary-antibodies-ab66597-6.jpg)
Anti-PP2A-alpha (methyl L309) antibody [2A10] (ab66597) at 1/100 dilution + Lystae prepared from mouse embryonic fibroblast cells at 25 µgSecondaryHRP-conjugatedsheep polyclonal to mouse IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![All lanes : Anti-PP2A-alpha (methyl L309) antibody [2A10] (ab66597) at 1/1000 dilutionLane 1 : No LCMT1 with 100% Licor Blocking Buffer for 30 minutes at 25°C.Lane 2 : LCMT1 at 0.02 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.Lane 3 : LCMT1 at 0.1 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.Lane 4 : LCMT1 at 0.5 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.SecondaryIRDye 800CW conjugated goat anti-mouse polyclonal at 1/5000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14639_PP2A-alpha-Primary-antibodies-ab66597-5.jpg)
All lanes : Anti-PP2A-alpha (methyl L309) antibody [2A10] (ab66597) at 1/1000 dilutionLane 1 : No LCMT1 with 100% Licor Blocking Buffer for 30 minutes at 25°C.Lane 2 : LCMT1 at 0.02 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.Lane 3 : LCMT1 at 0.1 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.Lane 4 : LCMT1 at 0.5 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.SecondaryIRDye 800CW conjugated goat anti-mouse polyclonal at 1/5000 dilutionPerformed under reducing conditions.
Product References
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Activation of AMP-activated protein kinase stimulates Na+,K+-ATPase activity in - Activation of AMP-activated protein kinase stimulates Na+,K+-ATPase activity in
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Microcystin-LR (MCLR) induces a compensation of PP2A activity mediated by alpha4 - Microcystin-LR (MCLR) induces a compensation of PP2A activity mediated by alpha4
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A stress survival response in retinal cells mediated through inhibition of the - A stress survival response in retinal cells mediated through inhibition of the
Finnegan S, Mackey AM, Cotter TG. Eur J Neurosci. 2010 Aug;32(3):322-34.
Generation of active protein phosphatase 2A is coupled to holoenzyme assembly. - Generation of active protein phosphatase 2A is coupled to holoenzyme assembly.
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Downregulation of protein phosphatase 2A carboxyl methylation and - Downregulation of protein phosphatase 2A carboxyl methylation and
Sontag E, Hladik C, Montgomery L, Luangpirom A, Mudrak I, Ogris E, White CL 3rd. J Neuropathol Exp Neurol. 2004 Oct;63(10):1080-91.
Altered expression levels of the protein phosphatase 2A ABalphaC enzyme are - Altered expression levels of the protein phosphatase 2A ABalphaC enzyme are
Sontag E, Luangpirom A, Hladik C, Mudrak I, Ogris E, Speciale S, White CL 3rd. J Neuropathol Exp Neurol. 2004 Apr;63(4):287-301.
A novel and essential mechanism determining specificity and activity of protein - A novel and essential mechanism determining specificity and activity of protein
Fellner T, Lackner DH, Hombauer H, Piribauer P, Mudrak I, Zaragoza K, Juno C, Ogris E. Genes Dev. 2003 Sep 1;17(17):2138-50.