Anti-PP2A-alpha (phospho Y307) antibody [E155]
| Name | Anti-PP2A-alpha (phospho Y307) antibody [E155] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32104 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | E155 |
| Applications | WB IHC-P ICC/IF IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Zebrafish |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PP2A-alpha |
| Description | Rabbit Monoclonal |
| Gene | PPP2CA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-PP2A-alpha (phospho Y307) antibody [E155] (ab32104) at 1/5000 dilutionLane 1 : untreated A431 cell lysateLane 2 : EGF treated A431 cell lysate (the specific EGF concentration and incubation time is confidential information)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/14642_WB%2520PP2A%2520alpha%2520(phospho%2520Y307).jpg)
All lanes : Anti-PP2A-alpha (phospho Y307) antibody [E155] (ab32104) at 1/5000 dilutionLane 1 : untreated A431 cell lysateLane 2 : EGF treated A431 cell lysate (the specific EGF concentration and incubation time is confidential information)
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Immunohistochemical analysis of PP2A alpha catalitic subunit (phospho Y307) expression in paraffin-embedded human brain, using 1/50 ab32104.
ab32104 (2µg/ml) staining PP2A alpha (phospho Y307) in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the islet of Langerhans.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab32104 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32104, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Enhanced insulin sensitivity associated with provision of mono and - Enhanced insulin sensitivity associated with provision of mono and
Nardi F, Lipina C, Magill D, Hage Hassan R, Hajduch E, Gray A, Hundal HS. PLoS One. 2014 Mar 14;9(3):e92255.
PP2A inhibition is a common event in colorectal cancer and its restoration using - PP2A inhibition is a common event in colorectal cancer and its restoration using
Cristobal I, Manso R, Rincon R, Carames C, Senin C, Borrero A, Martinez-Useros J, Rodriguez M, Zazo S, Aguilera O, Madoz-Gurpide J, Rojo F, Garcia-Foncillas J. Mol Cancer Ther. 2014 Apr;13(4):938-47.
Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and - Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and
Borghgraef P, Menuet C, Theunis C, Louis JV, Devijver H, Maurin H, Smet-Nocca C, Lippens G, Hilaire G, Gijsen H, Moechars D, Van Leuven F. PLoS One. 2013 Dec 23;8(12):e84442.
A novel function of Ataxin-1 in the modulation of PP2A activity is dysregulated - A novel function of Ataxin-1 in the modulation of PP2A activity is dysregulated
Sanchez I, Pinol P, Corral-Juan M, Pandolfo M, Matilla-Duenas A. Hum Mol Genet. 2013 Sep 1;22(17):3425-37.
Microcystin (-LR) induced testicular cell apoptosis via up-regulating - Microcystin (-LR) induced testicular cell apoptosis via up-regulating
Wang X, Chen Y, Zuo X, Ding N, Zeng H, Zou X, Han X. Food Chem Toxicol. 2013 Oct;60:309-17.
S100A8/A9 (calprotectin) negatively regulates G2/M cell cycle progression and - S100A8/A9 (calprotectin) negatively regulates G2/M cell cycle progression and
Khammanivong A, Wang C, Sorenson BS, Ross KF, Herzberg MC. PLoS One. 2013 Jul 9;8(7):e69395.
X-linked inhibitor of apoptosis protein (XIAP) regulation of cyclin D1 protein - X-linked inhibitor of apoptosis protein (XIAP) regulation of cyclin D1 protein
Cao Z, Zhang R, Li J, Huang H, Zhang D, Zhang J, Gao J, Chen J, Huang C. J Biol Chem. 2013 Jul 12;288(28):20238-47.
Antagonistic activities of the immunomodulator and PP2A-activating drug FTY720 - Antagonistic activities of the immunomodulator and PP2A-activating drug FTY720
Oaks JJ, Santhanam R, Walker CJ, Roof S, Harb JG, Ferenchak G, Eisfeld AK, Van Brocklyn JR, Briesewitz R, Saddoughi SA, Nagata K, Bittman R, Caligiuri MA, Abdel-Wahab O, Levine R, Arlinghaus RB, Quintas-Cardama A, Goldman JM, Apperley J, Reid A, Milojkovic D, Ziolo MT, Marcucci G, Ogretmen B, Neviani P, Perrotti D. Blood. 2013 Sep 12;122(11):1923-34.
Depletion of 4E-BP1 and regulation of autophagy lead to YXM110-induced anticancer - Depletion of 4E-BP1 and regulation of autophagy lead to YXM110-induced anticancer
Lai CY, Pan SL, Yang XM, Chang LH, Chang YL, Yang PC, Lee KH, Teng CM. Carcinogenesis. 2013 Sep;34(9):2050-60.
BAFF activates Erk1/2 promoting cell proliferation and survival by - BAFF activates Erk1/2 promoting cell proliferation and survival by
Liang D, Zeng Q, Xu Z, Zhang H, Gui L, Xu C, Chen S, Zhang S, Huang S, Chen L. Biochem Pharmacol. 2014 Jan 15;87(2):332-43.