All lanes : Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilutionLane 1 : U87-MG cell lysateLane 2 : Human fetal kidney lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/50000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Flow cytometric analysis of U87-MG cells (2% paraformaldehyde-fixed) labeling PPIC with ab184552 at 1/250 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.