All lanes : Anti-PPM1G antibody [EPR13362] (ab184184) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling PPM1G with ab184184 at 1/250 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.