All lanes : Anti-PPP2R5D antibody [EPR15617-50] (ab188325) at 1/50000 dilutionLane 1 : C6 cell lysateLane 2 : HepG2 cell lysateLane 3 : 293T cell lysateLane 4 : HeLa cell lysateLane 5 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PPP2R5D with ab188325 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling PPP2R5D with ab188325 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Western blot analysis of PPP2R5D in 293T cell lysate immunoprecipitated with ab188325 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1500 was used as secondary antibody.