Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122548 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122548, at 1:200, staining PPTC7 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
All lanes : Anti-PPTC7 antibody (ab122548) at 1/250 dilutionLane 1 : RT-4 cell lysateLane 2 : U-251 MG cell lysateLane 3 : Human plasma cell lysateLane 4 : Liver cell lysateLane 5 : Tonsil cell lysate