Flow cytometric analysis of NCI-H460 cells labeling PRAMEF8 with ab170710 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed and paraffin embedded Human melanoma tissue labeling PRAMEF8 with ab170710 at 1/10 diution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-PRAMEF8 antibody - C-terminal (ab170710) at 1/100 dilution + NCI-H460 cell line lysate at 35 µgdeveloped using the ECL technique