Anti-Prion protein PrP antibody [EP1802Y]
| Name | Anti-Prion protein PrP antibody [EP1802Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52604 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1802Y |
| Applications | WB FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues near the C-terminus of human Prion protein PrP |
| Description | Rabbit Monoclonal |
| Gene | PRNP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Prion protein PrP antibody [EP1802Y] (ab52604) at 1/10000 dilution + fetal brain lysate at 10 µgSecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/15845_ab52604wb.jpg)
Anti-Prion protein PrP antibody [EP1802Y] (ab52604) at 1/10000 dilution + fetal brain lysate at 10 µgSecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of brain glioma using ab52604 at a dilution of 1/100-1/250.
Overlay histogram showing SH-SY5Y cells stained with ab52604 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52604, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Live imaging of prions reveals nascent PrPSc in cell-surface, raft-associated - Live imaging of prions reveals nascent PrPSc in cell-surface, raft-associated
Rouvinski A, Karniely S, Kounin M, Moussa S, Goldberg MD, Warburg G, Lyakhovetsky R, Papy-Garcia D, Kutzsche J, Korth C, Carlson GA, Godsave SF, Peters PJ, Luhr K, Kristensson K, Taraboulos A. J Cell Biol. 2014 Feb 3;204(3):423-41.
Genetic prion disease: no role for the immune system in disease pathogenesis? - Genetic prion disease: no role for the immune system in disease pathogenesis?
Friedman-Levi Y, Binyamin O, Frid K, Ovadia H, Gabizon R. Hum Mol Genet. 2014 Aug 1;23(15):4134-41.
Targeted knock-down of cellular prion protein expression in myelinating Schwann - Targeted knock-down of cellular prion protein expression in myelinating Schwann
Halliez S, Chesnais N, Mallucci G, Vilotte M, Langevin C, Jaumain E, Laude H, Vilotte JL, Beringue V. J Gen Virol. 2013 Jun;94(Pt 6):1435-40.
Highly neurotoxic monomeric alpha-helical prion protein. - Highly neurotoxic monomeric alpha-helical prion protein.
Zhou M, Ottenberg G, Sferrazza GF, Lasmezas CI. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3113-8. doi:
Early mechanisms of pathobiology are revealed by transcriptional temporal - Early mechanisms of pathobiology are revealed by transcriptional temporal
Majer A, Medina SJ, Niu Y, Abrenica B, Manguiat KJ, Frost KL, Philipson CS, Sorensen DL, Booth SA. PLoS Pathog. 2012;8(11):e1003002.
Pharmacological prion protein silencing accelerates central nervous system - Pharmacological prion protein silencing accelerates central nervous system
Hu W, Nessler S, Hemmer B, Eagar TN, Kane LP, Leliveld SR, Muller-Schiffmann A, Gocke AR, Lovett-Racke A, Ben LH, Hussain RZ, Breil A, Elliott JL, Puttaparthi K, Cravens PD, Singh MP, Petsch B, Stitz L, Racke MK, Korth C, Stuve O. Brain. 2010 Feb;133(Pt 2):375-88.
Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine - Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine
Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M. Proteomics Clin Appl. 2010 Apr;4(4):416-25.
Prion protein expression and the M129V polymorphism of the PRNP gene in patients - Prion protein expression and the M129V polymorphism of the PRNP gene in patients
Antonacopoulou AG, Palli M, Marousi S, Dimitrakopoulos FI, Kyriakopoulou U, Tsamandas AC, Scopa CD, Papavassiliou AG, Kalofonos HP. Mol Carcinog. 2010 Jul;49(7):693-9.
Age-related cellular copper dynamics in the fungal ageing model Podospora - Age-related cellular copper dynamics in the fungal ageing model Podospora
Scheckhuber CQ, Grief J, Boilan E, Luce K, Debacq-Chainiaux F, Rittmeyer C, Gredilla R, Kolbesen BO, Toussaint O, Osiewacz HD. PLoS One. 2009;4(3):e4919.
Aggregation and amyloid fibril formation induced by chemical dimerization of - Aggregation and amyloid fibril formation induced by chemical dimerization of
Roostaee A, Cote S, Roucou X. J Biol Chem. 2009 Nov 6;284(45):30907-16.