Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)]

• Supplier: Abcam
• Catalog: ab187660
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1044(2)
• Applications: IHC-P FC WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
• Description: Rabbit Monoclonal
• Gene: PKN3
• Conjugate: Unconjugated

Product images

All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660) at 1/10000 dilutionLane 1 : Lysate from Hela cells treated with Okadaic acid + Calyculin ALane 2 : Untreated Hela cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) with ab187660 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) with ab187660 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Flow cytometric analysis of permeabilized HeLa cells, untreated (green) or Okadaic acid + Calyculin A-treated (red) using ab187660 at 1/280 dilution, and Okadaic acid + Calyculin A-treated HeLa cells using ab187660 preincubated with phospho-PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) peptide (blue) or non-phospho-PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) peptide (orange). Cells were fixed with 2% paraformaldehyde. Secondary antibody was a Goat anti IgG (FITC) at 1/150 dilution.