Anti-PRKCBP1 antibody [EPR16924]

• Supplier: Abcam
• Catalog: ab201452
• Prices: $99.00, $347.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16924
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PRKCBP1 aa 650-750
• Description: Rabbit Monoclonal
• Gene: ZMYND8
• Conjugate: Unconjugated

Product images

All lanes : Anti-PRKCBP1 antibody [EPR16924] (ab201452) at 1/1000 dilutionLane 1 : HEK293 (Human epithelial cells from embryonic kidney) whole cell lysateLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 3 : Human fetal kidney lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRKCBP1 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.Note: Nuclear staining on Human pancreas tissue is observed.

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling PRKCBP1 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.Note: Nuclear staining on Human cervix carcinoma tissue is observed.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex  tissue labeling PRKCBP1 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.Note: Nuclear staining on Human cerebral cortex tissue was observed.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human epithelial cells from embryonic kidney) cells labeling PRKCBP1 with ab201452 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing nuclear staining on HEK293 cell line.The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:1. ab201452 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Flow cytometry analysis of HEK293 (Human epithelial cells from embryonic kidney) cells labeling PRKCBP1 using ab201452 at 1/150 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).