Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PRKRIR with ab70334 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-PRKRIR antibody (ab70334) at 0.04 µg/mlLane 1 : whole cell lysate of HeLa cells at 50 µgLane 2 : whole cell lysate of HeLa cells at 15 µgLane 3 : whole cell lysate of HeLa cells at 5 µgLane 4 : whole cell lysate of NIH3T3 cells at 50 µg
IP/WB - 1 mg whole cell lysate of HeLa cells used, and 1/4 of the reaction loaded. Lane 1: ab70333 used at 3µg/ml lysate for IP. Lane 2: ab70334 used at 3µg/ml lysate for IP. Lane 3: IgG control. ab70334 used at at 1µg/ml for the subsequent WB for all lanes. Detection: Chemiluminescence with an exposure time of 30 seconds.