Anti-PRMT5 antibody
| Name | Anti-PRMT5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31751 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Human, Xenopus, Zebrafish |
| Antigen | Synthetic peptide derived from within residues 600 to the C-terminus of Human PRMT5 |
| Description | Rabbit Polyclonal |
| Gene | PRMT5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Image courtesy of Human Protein Atlas ab31751 staining PRMT5 in human prostate, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab31751 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab31751 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
All lanes : Anti-PRMT5 antibody (ab31751) at 1 µg/mlLane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Enhanced arginine methylation of programmed cell death 4 protein during nutrient - Enhanced arginine methylation of programmed cell death 4 protein during nutrient
Fay MM, Clegg JM, Uchida KA, Powers MA, Ullman KS. J Biol Chem. 2014 Jun 20;289(25):17541-52.
PRMT5 is upregulated in malignant and metastatic melanoma and regulates - PRMT5 is upregulated in malignant and metastatic melanoma and regulates
Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, Sif S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, Lesinski GB. PLoS One. 2013 Sep 30;8(9):e74710.
Menin directly represses Gli1 expression independent of canonical Hedgehog - Menin directly represses Gli1 expression independent of canonical Hedgehog
Gurung B, Feng Z, Hua X. Mol Cancer Res. 2013 Oct;11(10):1215-22.
Proteomic analysis of murine Piwi proteins reveals a role for arginine - Proteomic analysis of murine Piwi proteins reveals a role for arginine
Vagin VV, Wohlschlegel J, Qu J, Jonsson Z, Huang X, Chuma S, Girard A, Sachidanandam R, Hannon GJ, Aravin AA. Genes Dev. 2009 Aug 1;23(15):1749-62.