Anti-PRMT5 antibody [EPR5772]

Name	Anti-PRMT5 antibody [EPR5772]
Supplier	Abcam
Catalog	ab109451
Prices	$388.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR5772
Applications	WB IHC-P FC ICC/IF ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PRMT5 aa 50-150
Description	Rabbit Monoclonal
Gene	PRMT5
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-PRMT5 antibody [EPR5772] (ab109451) at 1/10000 dilution (purified) + HeLa cell lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-PRMT5 antibody [EPR5772] (ab109451) at 1/50000 dilution (purified)Lane 1 : HEK293 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-PRMT5 antibody [EPR5772] (ab109451) at 1/10000 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue sections labelling PRMT5 with purified ab109451 at 1/100. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labelling PRMT5 with purified ab109451 at 1/100. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PRMT5 (green) with purified ab109451 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/50) and secondary antibody Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/400).

ICC/IF image of ab109451 (unpurified) stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109451, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing HeLa cells stained with ab109451 (unpurified, red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109451, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Equilibrium disassociation constant (KD)Click here to learn more about KD

Product References

Menin directly represses Gli1 expression independent of canonical Hedgehog - Menin directly represses Gli1 expression independent of canonical Hedgehog

Gurung B, Feng Z, Hua X. Mol Cancer Res. 2013 Oct;11(10):1215-22.

Generation of human induced pluripotent stem cells using epigenetic regulators - Generation of human induced pluripotent stem cells using epigenetic regulators

Goyal A, Chavez SL, Reijo Pera RA. PLoS One. 2013 Dec 12;8(12):e82838.