All lanes : Anti-PRMT7 antibody [EPR13495] (ab181214) at 1/2000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : 293 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of HeLa cells (-20℃ acetone-fixed) labeling PRMT7 with ab181214 at 1/250 dilution (red). Goat anti rabbit IgG (Alexa Fluor® 555) was used as a secondary antibody, at a dilution of 1/200. Counter stain: Dapi (blue).