Anti-Prolactin antibody (ab103617) at 1/500 dilution + Mouse testis tissue lysate at 50 µg
All lanes : Anti-Prolactin antibody (ab103617) at 1/500 dilutionLane 1 : Prolactin transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.
Anti-Prolactin antibody (ab103617) at 1/500 dilution + Human Prolactin full length protein (ab51703) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 90 seconds