Anti-Prostaglandin E Receptor EP2 antibody [EPR8030(B)]

• Supplier: Abcam
• Catalog: ab167171
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR8030(B)
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to a cytoplasmic region near the C terminal of Human Prostaglandin E Receptor EP2 (UniProt: P43116)
• Description: Rabbit Monoclonal
• Gene: PTGER2
• Conjugate: Unconjugated

Product images

ab167171 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab167171 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-Prostaglandin E Receptor EP2 antibody [EPR8030(B)] (ab167171) at 1/1000 dilutionLane 1 : Human placenta lysatesLane 2 : Human fetal lung lysatesLane 3 : JAR cell lysatesLane 4 : WI38 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Prostaglandin E Receptor EP2 with ab167171 at 1/250 dilution.