Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Prostaglandin E Synthase with ab180589 at 1/400 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG used as Isotype control (green).
All lanes : Anti-Prostaglandin E Synthase antibody [EPR13765] (ab180589) at 1/1000 dilutionLane 1 : Lysate of NIH 3T3 cells with Immunizing peptideLane 2 : Lysate of NIH 3T3 cellsLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Prostaglandin E Synthase antibody [EPR13765] (ab180589) at 1/1000 dilutionLane 1 : NIH 3T3 cell line lysateLane 2 : Jurkat cell line lysateLane 3 : HeLa cell line lysateLane 4 : A549 cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution