Anti-Proteasome 20S alpha + beta antibody

• Supplier: Abcam
• Catalog: ab22673
• Prices: $400.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF IHC-F WB IP ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Yeast
• Antigen: Tissue/ cell preparation: Proteasomal preparation isolated from human red blood cells
• Description: Rabbit Polyclonal
• Gene: PSMA5
• Conjugate: Unconjugated

Product images

Anti-Proteasome 20S alpha + beta antibody (ab22673) at 1/1000 dilutiondeveloped using the ECL technique

All lanes : Anti-Proteasome 20S alpha + beta antibody (ab22673) at 1/2000 dilutionLane 1 : Whole tissue lysate prepared from rat prefrontal cortexLane 2 : Whole tissue lysate prepared from rat prefrontal cortexLane 3 : Whole tissue lysate prepared from rat prefrontal cortexLane 4 : Whole tissue lysate prepared from rat prefrontal cortexLane 5 : Whole tissue lysate prepared from rat prefrontal cortexLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG-HRP at 1/5000 dilutiondeveloped using the ECL technique

ab22673 staining human Hela cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in 0.1% Triton and blocked in 3% BSA for 15 minutes at room temperature. The primary antibody was diluted 1/2000 (3% BSA) and incubated with sample for 1 hour. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit, diluted 1/1000 was used as secondary.See Abreview

Immunofluorescence analysis of HeLa cells, staining Proteasome 20S alpha + beta with ab22673. Cells were fixed with formaldehyde and permeabilized using 0.1% Triton X-100. Samples were incubated with primary antibody (1/1000) fr 1 hour at room temperature. An AlexaFluor®555-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

Product References

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