Luminograph of human placental proteasome preparation after 2D PADE followed by blotting onto nitrocellulose and probing with ab22666 (1/1000). ECL exposure , 1 minute exposure).
![All lanes : Anti-Proteasome 20S alpha 2 antibody [MCP21] (ab22666) at 1/1000 dilutionLane 1 : 40ug SU-DHL1 whole cell lysateLane 2 : 40ug Karpas 299 whole cell lysateLane 3 : 40ug JB-6 whole cell lysateLane 4 : 40ug SUP-M2 whole cell lysateLane 5 : 40ug Mac-1 whole cell lysateLane 6 : 40ug Jurkat whole cell lysateSecondaryBiotinylated polyclonal anti-mouse antibody.developed using the ECL techniquePerformed under reducing conditions.Observed band size : 27 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an Abreview submitted by Dr Carlo ViglianiSee Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/16713_ab22666-2.jpg)
All lanes : Anti-Proteasome 20S alpha 2 antibody [MCP21] (ab22666) at 1/1000 dilutionLane 1 : 40ug SU-DHL1 whole cell lysateLane 2 : 40ug Karpas 299 whole cell lysateLane 3 : 40ug JB-6 whole cell lysateLane 4 : 40ug SUP-M2 whole cell lysateLane 5 : 40ug Mac-1 whole cell lysateLane 6 : 40ug Jurkat whole cell lysateSecondaryBiotinylated polyclonal anti-mouse antibody.developed using the ECL techniquePerformed under reducing conditions.Observed band size : 27 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an Abreview submitted by Dr Carlo ViglianiSee Abreview
![Overlay histogram showing HeLa cells stained with ab22666 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22666, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/16714_Proteasome-20S-alpha-2-Primary-antibodies-ab22666-1.jpg)
Overlay histogram showing HeLa cells stained with ab22666 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22666, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.