Anti-Proteasome 20S beta 6 antibody
| Name | Anti-Proteasome 20S beta 6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3331 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF |
| Species Reactivities | Mouse, Bovine, Human, Rat, Xenopus, Zebrafish |
| Antigen | Synthetic peptide corresponding to Human Proteasome 20S beta 6 aa 41-58 |
| Description | Rabbit Polyclonal |
| Gene | PSMB6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
All lanes : Anti-Proteasome 20S beta 6 antibody (ab3331) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : BAEC cell lysateLysates/proteins at 25 µg per lane.