Anti-Proteasome 20S LMP2 antibody
| Name | Anti-Proteasome 20S LMP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3328 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Human, Rat, Bovine |
| Antigen | Synthetic peptide corresponding to Human Proteasome 20S LMP2 aa 206-219 |
| Description | Rabbit Polyclonal |
| Gene | PSMB9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.
ab3328 (1µg/ml) staining Proteasome 20S LMP2 in human heart using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and cytoplasmic cell compartments.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue (neurons in HIV encephalitis) sections labelling LMP2 with ab3328 at 1/1000. Immunofluorescence staining for LMP2 shows colocalization with the neuronal marker NeuN. LMP2 is present in granular deposits in the parikaryon. Scale bar = 10 μm.
Product References
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Impairment of immunoproteasome function by beta5i/LMP7 subunit deficiency results - Impairment of immunoproteasome function by beta5i/LMP7 subunit deficiency results
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