Anti-Proteasome 20S LMP2 antibody
| Name | Anti-Proteasome 20S LMP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab42987 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Human, Rat, Bovine, Dog, Pig |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Proteasome 20S LMP2 |
| Description | Rabbit Polyclonal |
| Gene | PSMB9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Proteasome 20S LMP2 antibody (ab42987) at 0.5 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab42987 stained MEF1 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42987, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
IHC image of ab42987 staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42987, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome - TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome
Pla A, Pascual M, Renau-Piqueras J, Guerri C. Cell Death Dis. 2014 Feb 20;5:e1066.
c-Myc and EBV-LMP1: two opposing regulators of the HLA class I antigen - c-Myc and EBV-LMP1: two opposing regulators of the HLA class I antigen
Tudor CS, Dawson CW, Eckhardt J, Niedobitek G, Buttner AC, Seliger B, Hartmann A, Buettner M. Br J Cancer. 2012 Jun 5;106(12):1980-8.
Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages - Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages
Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W. J Immunol. 2011 Jul 15;187(2):748-59.
Unexpected role for the immunoproteasome subunit LMP2 in antiviral humoral and - Unexpected role for the immunoproteasome subunit LMP2 in antiviral humoral and
Hensley SE, Zanker D, Dolan BP, David A, Hickman HD, Embry AC, Skon CN, Grebe KM, Griffin TA, Chen W, Bennink JR, Yewdell JW. J Immunol. 2010 Apr 15;184(8):4115-22.