All lanes : Anti-EXTL1 antibody - C-terminal (ab174172) at 1/100000 dilutionLane 1 : Ramos cell lysateLane 2 : A431 cell lysateLane 3 : Raji cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde fixed HL60 cells labeling Proteasome 20S LMP2 using ab184172 at 1/100 dilution (red). A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).
Immunoprecipitation analysis of Raji cells labeling Proteasome 20S LMP2 using ab184172 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Raji lysates. Blocking/dilution buffer and concentration: 5% NFDM/TBST.