All lanes : Anti-Proteasome 20S LMP2 antibody [EPR15129(B)] (ab190350) at 1/2000 dilutionLane 1 : Raji cell lysatesLane 2 : Ramos cell lysatesLane 3 : Human fetal thymus lysatesLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1000 developed using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human spleen tissue labeling Proteasome 20S LMP2 with ab190350 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.
Immunofluorescence analysis of, paraformaldehyde-fixed, Jurkat cells labeling Proteasome 20S LMP2 with ab190350 at a 1/50 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining. Negative controls also shown.
Flow cytometry analysis of 2% paraformaldehyde-fixed Raji cells labeling Proteasome 20S LMP2 with ab190350 at a 1/110dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.