All lanes : Anti-Proteasome 20S LMP2 antibody [EPR15130(B)] (ab193357) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : Ramos cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat cells labeling Proteasome 20S LMP2 with ab193357 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue).