![Overlay histogram showing MCF7 cells stained with ab119959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119959, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/16833_ab119959-7-ab119959FC.jpg)
Overlay histogram showing MCF7 cells stained with ab119959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119959, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-Proteasome 20S LMP7 antibody [1A5] (ab119959) at 1/500 dilution + Recombinant Proteasome 20S LMP7 protein](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/16834_Proteasome-20S-LMP7-Primary-antibodies-ab119959-1.jpg)
Anti-Proteasome 20S LMP7 antibody [1A5] (ab119959) at 1/500 dilution + Recombinant Proteasome 20S LMP7 protein
![All lanes : Anti-Proteasome 20S LMP7 antibody [1A5] (ab119959) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : A431 cell lysateLane 4 : Raji cell lysateLane 5 : MOLT4 cell lysateLane 6 : PC12 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/16835_Proteasome-20S-LMP7-Primary-antibodies-ab119959-2.jpg)
All lanes : Anti-Proteasome 20S LMP7 antibody [1A5] (ab119959) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : A431 cell lysateLane 4 : Raji cell lysateLane 5 : MOLT4 cell lysateLane 6 : PC12 cell lysate
ab119959 at 1/200 dilution staining Proteasome 20S LMP7 in Paraffin-embedded Human intima cancer tissue by Immunohistochemistry followed by DAB staining.
ab119959 at 1/200 dilution staining Proteasome 20S LMP7 in Paraffin-embedded Human colon tissue by Immunohistochemistry followed by DAB staining.
ab119959 at 1/200 dilution staining Proteasome 20S LMP7 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA, showing ab119959.