Anti-Proteasome 20S LMP7 antibody [EPR14482(B)]

• Supplier: Abcam
• Catalog: ab180606
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14482(B)
• Applications: FC IHC-P WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Proteasome 20S LMP7 aa 200 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: PSMB8
• Conjugate: Unconjugated

Product images

Western blot analysis of Raji lysate immunoprecipitated with ab180606 at 1/40 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG used at 1/1500 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling Proteasome 20S LMP7 with ab180606 at 1/150 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunofluorecenct analysis of 4% paraformaldehyde-fixed HeLa cells labeling Proteasome 20S LMP7 with ab180606 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (left panel). Dapi staining (right panel).

Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Proteasome 20S LMP7 with ab180606 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Proteasome 20S LMP7 with ab180606 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

All lanes : Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] (ab180606) at 1/10000 dilutionLane 1 : Raji cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : U937 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution