Detection of Human Protective protein/Cathepsin A (PPCA) by Western Blot of Immunoprecipitate. 293 whole cell lysate loaded, ab181129 used for blotting immunoprecipitated Protective protein/Cathepsin A (PPCA) at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
All lanes : Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] (ab181129) at 1/20000 dilutionLane 1 : 293 lysateLane 2 : HeLa lysateLane 3 : Jurkat lysateLane 4 : HepG2 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution