ab76781 staining PRSS2 in Human primary motor cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 2 hours at 20°C; antigen retrieval was by heat mediation in tris EDTA, pH9.2. Samples were incubated with primary antibody (1/200 in 2% serum, 0.5% Triton X-100 in PBS) for 20 hours at 20°C. An undiluted Biotin-conjugated Sheep anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview
Anti-PRSS2 antibody (ab76781) at 1/500 dilution + Human pancreas tissue lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilutiondeveloped using the ECL technique
All lanes : Anti-PRSS2 antibody (ab76781) at 1/500 dilutionLane 1 : PRSS2 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilutiondeveloped using the ECL technique