![Lane 1 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 0.0825 µg/mlLanes 2 - 3 : Anti-PSD95 (phospho T19) antibody (ab16496) at 1 µg/mlLane 1 : mouse forebrainLane 2 : mouse forebrainLane 3 : mouse forebrain with Mouse PSD95 (phospho T19) peptide (ab17167) at 1 µg/mlLysates/proteins at 20 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17647_ab16496_1.jpg)
Lane 1 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 0.0825 µg/mlLanes 2 - 3 : Anti-PSD95 (phospho T19) antibody (ab16496) at 1 µg/mlLane 1 : mouse forebrainLane 2 : mouse forebrainLane 3 : mouse forebrain with Mouse PSD95 (phospho T19) peptide (ab17167) at 1 µg/mlLysates/proteins at 20 µg per lane.
IHC image of PSD95 (Phospho T19) staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16496, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
![PSD95 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to PSD95 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16496.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 105kDa, non specific band - 96kDa: We are unsure as to the identity of this extra band; PSD95](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17649_PSD95-Primary-antibodies-ab16496-24.jpg)
PSD95 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to PSD95 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16496.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 105kDa, non specific band - 96kDa: We are unsure as to the identity of this extra band; PSD95