Anti-PSG9 antibody
| Name | Anti-PSG9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64425 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | A region within synthetic peptide: VYSNASLLIQ NVTRKDAGTY TLHIIKRGDE TREEIRHFTF TLYLETPKPY , corresponding to amino acids 101-150 of Human PSG9 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | PSG9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PSG9 antibody (ab64425) at 0.25 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab64425 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64425, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.