Anti-PSMA antibody [YPSMA-1]
| Name | Anti-PSMA antibody [YPSMA-1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab19071 |
| Prices | $390.00 |
| Sizes | 250 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | YPSMA-1 |
| Applications | ELISA WB IHC-P IHC-F ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Crude membrane protein preparation from pooled prostate malignant carcinoma from China |
| Description | Mouse Monoclonal |
| Gene | FOLH1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing LnCAP cells stained with ab19071 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19071, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in LnCAP cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17802_ab19071-1-ab19071FC.jpg)
Overlay histogram showing LnCAP cells stained with ab19071 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19071, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in LnCAP cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
PSMA, EpCAM, VEGF and GRPR as imaging targets in locally recurrent prostate - PSMA, EpCAM, VEGF and GRPR as imaging targets in locally recurrent prostate
Rybalov M, Ananias HJ, Hoving HD, van der Poel HG, Rosati S, de Jong IJ. Int J Mol Sci. 2014 Apr 10;15(4):6046-61.
Identification of a LNCaP-specific binding peptide using phage display. - Identification of a LNCaP-specific binding peptide using phage display.
Qin B, Tai W, Shukla RS, Cheng K. Pharm Res. 2011 Oct;28(10):2422-34.