Western blot analysis on immunoprecipitation pellet from (Lane 1) HeLa cell lysate or (Lane 2) 1X PBS (negative control) using ab171969 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-PSMC3 antibody [EPR12152] - N-terminal (ab171969) at 1/1000 dilutionLane 1 : Human placenta lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labeled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PSMC3 using ab171969 at a 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling PSMC3 using ab171969 at a 1/50 dilution.
Immunofluorescence analysis of 293 cells labeling PSMC3 using ab171969 at a 1/250 dilution.
Flow cytometric analysis of permeabilized 293 cells, labeling PSMC3 using ab171969 at a 1/10 dilution (red) or a rabbit IgG negative control (green).